pEB1deltaC-2xEGFP shRNA resistant
(Plasmid #37925)

• Depositing Lab: Torsten Wittmann
• Publication: Gierke et al Curr Biol. 2012 May 8;22(9):753-62. Epub 2012 Apr 5.

Backbone

• Vector backbone: pEGFP-N1
• Total vector size (bp): 4733
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EB1
• Species: H. sapiens (human)
• Mutation: deletion after aa248, TTCCTGT=>TCCCGGT
• Entrez Gene: MAPRE1 (a.k.a. EB1)
• Promoter: CMV
• Tags / Fusion Proteins:
  • EGFP (C terminal on insert)
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This construct is resistant to shRNA #3.

EB1 was inserted into pEGFP-N1 via the SacI/SmaI restriction sites. The second EGFP, was then inserted via the SmaI/AgeI sites.