MSCV-N BARF1
(Plasmid #37922)

• Depositing Lab: Karl Munger
• Publication: Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Backbone

• Vector backbone: MSCV-N FLAGHA
• Backbone size w/o insert (bp): 8162
• Total vector size (bp): 7325
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: BARF1
• Species: H. Herpesvirus 4 (EBV)
• Insert Size (bp): 663
• GenBank ID: YP_401719.1
• Entrez Gene: BARF1 (a.k.a. HHV4_BARF1.2)
• Promoter: PKG
• Tags / Fusion Proteins:
  • Flag (N terminal on backbone)
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: LXSN 5'
• 3′ sequencing primer: MSCV-N seq. rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: E. Kieff lab
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, leaving additional amino acid residues [PTFLYKVVR] fused to the C-terminus of translated protein.

Though there are some discrepancies between the depositor sequence and Addgene's quality control sequence, they are not known to affect plasmid function.