Skip to main content
Addgene

MSCV-N BKV ER
(Plasmid #37864)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37864 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    MSCV-N FLAGHA
  • Backbone size w/o insert (bp) 8162
  • Total vector size (bp) 9085
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    BKV ER
  • Alt name
    BK polyomavirus Large T (truncated)
  • Alt name
    BK polymavirus Small T
  • Alt name
    BKPyVgp5 and BKPyVgp6
  • Species
    BK polyomavirus
  • Insert Size (bp)
    1000
  • Entrez Gene
    BKPyVgp5 (a.k.a. BKPyVgp5)
  • Entrez Gene
    BKPyVgp6 (a.k.a. BKPyVgp6)
  • Promoter PKG
  • Tags / Fusion Proteins
    • Flag (N terminal on backbone)
    • HA (N terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    James DeCaprio lab

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There is a splice site in the DNA to make a truncated Lg T (348bp), as well as a full length small T, both tagged at N-terminal end.
The small and Lg T seqences overlap for the first 242 bp. (genome entry, BKV, strain UT: https://www.ncbi.nlm.nih.gov/nucleotide/83415612?report=genbank&log$=nuclalign&blast_rank=4&RID=53X3T5WA016 )
Both proteins are seen when this construct is expressed in cells.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV-N BKV ER was a gift from Karl Munger (Addgene plasmid # 37864 ; http://n2t.net/addgene:37864 ; RRID:Addgene_37864)

  • For your References section:

    Interpreting cancer genomes using systematic host network perturbations by tumour virus proteins. Rozenblatt-Rosen O, Deo RC, Padi M, Adelmant G, Calderwood MA, Rolland T, Grace M, Dricot A, Askenazi M, Tavares M, Pevzner SJ, Abderazzaq F, Byrdsong D, Carvunis AR, Chen AA, Cheng J, Correll M, Duarte M, Fan C, Feltkamp MC, Ficarro SB, Franchi R, Garg BK, Gulbahce N, Hao T, Holthaus AM, James R, Korkhin A, Litovchick L, Mar JC, Pak TR, Rabello S, Rubio R, Shen Y, Singh S, Spangle JM, Tasan M, Wanamaker S, Webber JT, Roecklein-Canfield J, Johannsen E, Barabasi AL, Beroukhim R, Kieff E, Cusick ME, Hill DE, Munger K, Marto JA, Quackenbush J, Roth FP, DeCaprio JA, Vidal M. Nature. 2012 Jul 26;487(7408):491-5. 10.1038/nature11288 PubMed 22810586
Related items:
Commonly requested with: