MSCV-N SV40 ST
(Plasmid #37858)

• Depositing Lab: Karl Munger
• Publication: Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Backbone

• Vector backbone: MSCV-N FLAGHA
• Backbone size w/o insert (bp): 8162
• Total vector size (bp): 6914
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: SV40 ST
• Alt name: Simian virus 40 Small T
• Species: Simian Virus 40
• Insert Size (bp): 252
• GenBank ID: YP_003708383.1
• Entrez Gene: SV40gp7 (a.k.a. SV40gp7)
• Promoter: PKG
• Tags / Fusion Proteins:
  • Flag (N terminal on backbone)
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: LXSN 5'
• 3′ sequencing primer: MSCV-N seq. rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: James DeCaprio lab
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, leaving additional amino acid residues [STFLYKVVR] fused to the C-terminus of translated protein.