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Addgene

3xERRE/ERE-luciferase
(Plasmid #37852)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37852 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3-Promoter
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5010
  • Total vector size (bp) 5098
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    3 copies of ERRE/ERE upstream of luciferase
  • Species
    codon-modified firefly (Photinus pyralis)
  • Insert Size (bp)
    88
  • Promoter SV40

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer RVprimer3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3xERRE/ERE-luciferase was a gift from Rebecca Riggins (Addgene plasmid # 37852 ; http://n2t.net/addgene:37852 ; RRID:Addgene_37852)

  • For your References section:

    ERK/MAPK regulates ERRgamma expression, transcriptional activity, and receptor-mediated Tamoxifen resistance in ER+ breast cancer. Heckler MM, Thakor H, Schafer CC, Riggins RB. FEBS J. 2014 Mar 29. doi: 10.1111/febs.12797. 10.1111/febs.12797 PubMed 24684682
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