pAcGP67A-rCL1-HormR-GAIN
(Plasmid
#37847)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 37847 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAcGP67A
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Backbone manufacturerBD Biosciences
- Backbone size w/o insert (bp) 9761
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Vector typeBaculovirus Transfer Vectors
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCL1
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SpeciesR. norvegicus (rat)
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Insert Size (bp)1167
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MutationHormR and GAIN domains only (aa460–849
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Entrez GeneAdgrl1 (a.k.a. CL1BA, Lphn1)
- Promoter Polyhedrin
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Tag
/ Fusion Protein
- 8xHIS (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Xba1 (not destroyed)
- 3′ cloning site Not1 (not destroyed)
- 5′ sequencing primer polyhedrin-fwd
- 3′ sequencing primer polyhedrin-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAcGP67A-rCL1-HormR-GAIN was a gift from Axel Brunger (Addgene plasmid # 37847 ; http://n2t.net/addgene:37847 ; RRID:Addgene_37847) -
For your References section:
A novel evolutionarily conserved domain of cell-adhesion GPCRs mediates autoproteolysis. Arac D, Boucard AA, Bolliger MF, Nguyen J, Soltis SM, Sudhof TC, Brunger AT. EMBO J. 2012 Feb 14;31(6):1364-78. doi: 10.1038/emboj.2012.26. 10.1038/emboj.2012.26 PubMed 22333914