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Addgene

pAct1IHPT-4
(Plasmid #37844)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37844 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pILTAB222
  • Backbone manufacturer
    Sivamani et al PCR 1996
  • Total vector size (bp) 5870
  • Vector type
    Plant transformation
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Actin promoter and intron
  • Alt name
    Act1
  • Species
    Oryza sativa
  • Tags / Fusion Proteins
    • HPT (Hygromycin phosphotransferase) (C terminal on backbone)
    • nos 3' terminator (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)
  • 5′ sequencing primer Hygro-R
  • 3′ sequencing primer Hygro-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There are several sequence discrepancies between Addgene's quality control sequence and the available NCBI reference sequence for actin promoter. These differences should not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAct1IHPT-4 was a gift from Peggy Lemaux (Addgene plasmid # 37844 ; http://n2t.net/addgene:37844 ; RRID:Addgene_37844)

  • For your References section:

    Transformation of recalcitrant barley cultivars through improvement of regenerability and decreased albinism. Myeong-Je Chor, Wen Jiang, Peggy G. Lemaux. Volume 138, Issue 2, 23 November 1998, Pages 229–244 10.1016/S0168-9452(98)00162-9
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