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Addgene

pAAV-CAG-tdTomato
(Plasmid #37811)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37811 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    AAV with CAG promoter
  • Backbone manufacturer
    Scott Sternson
  • Backbone size w/o insert (bp) 4722
  • Vector type
    Mammalian Expression, AAV ; Adeno-associated virus

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Use recombinase-free E. coli (Stbl3, XL-10, Sure2 and DH5a et.al), grow at 30C
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    tdTomato
  • Species
    engineered from red fluorescent protein from coral
  • Insert Size (bp)
    1431
  • Mutation
    N/A
  • Promoter CAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer cggcttctggcgtgtgac
  • 3′ sequencing primer aaagagacagcaaccagg
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PLEASE CONTACT ED BOYDEN ([email protected]) FOR DETAILS ON THIS REAGENT AND FURTHER REAGENTS IN THIS LINE.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CAG-tdTomato was a gift from Edward Boyden (Addgene plasmid # 37811)
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