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Addgene

NUMB2-eGFP-M107K
(Plasmid #37803)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37803 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    M107
  • Backbone manufacturer
    Beyer et al., J Virol 2002
  • Backbone size w/o insert (bp) 7309
  • Total vector size (bp) 9124
  • Modifications to backbone
    Kozak consensus sequence
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NUMB isoform 2
  • Alt name
    NUMB2
  • Alt name
    NUMB
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1812
  • GenBank ID
    NM_001005744.1
  • Entrez Gene
    NUMB (a.k.a. C14orf41, S171, c14_5527)
  • Promoter pCMV
  • Tag / Fusion Protein
    • eGFP (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NUMB2-eGFP-M107K was a gift from Rolf Bjerkvig (Addgene plasmid # 37803 ; http://n2t.net/addgene:37803 ; RRID:Addgene_37803)

  • For your References section:

    NUMB does not impair growth and differentiation status of experimental gliomas. Euskirchen P, Skaftnesmo KO, Huszthy PC, Brekka N, Bjerkvig R, Jacobs AH, Miletic H. Exp Cell Res. 2011 Dec 10;317(20):2864-73. Epub 2011 Sep 12. 10.1016/j.yexcr.2011.09.002 PubMed 21939656
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