PIGAZFN-R2
(Plasmid #37800)

• Depositing Lab: Linzhao Cheng
• Publication: Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Backbone

• Vector backbone: pST1374
• Backbone manufacturer: Keith Joung lab @ MGH/HMS
• Backbone size w/o insert (bp): 5709
• Total vector size (bp): 5973
• Modifications to backbone: The backbone was digested using XbaI/BamHI to insert the zinc finger sequence.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PIGA zinc finger R2
• Species: Synthetic
• Insert Size (bp): 264
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The insert and backbone were from Dr. Keith Joung's lab in Massachusetts General Hospital/Harvard Medical School.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please also cite the original article from Joung lab describing the construction of zinc finger nuclease:
Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, et al. Rapid “open-source” engineering of customized zinc-finger nucleases for highly efficient gene modification. Mol Cell. 2008;31:294–301.