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Addgene

pUASP YFP Rab19 DN
(Plasmid #37721)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37721 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUASP
  • Backbone size w/o insert (bp) 9939
  • Vector type
    Insect Expression ; Drosophila

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rab19
  • Species
    D. melanogaster (fly)
  • Mutation
    T35N (dominant negative)
  • Entrez Gene
    Rab19 (a.k.a. Dmel_CG7062, AAF50452, CG7062, DRABR3, DRabRP3, DmRab19, Dmel\CG7062, Rab-RP3, Rab-r3, Rab19/RP3, Rab19s, rab19)
  • Promoter GAL4
  • Tag / Fusion Protein
    • YFP (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer pUASp-3 5'-AGGTTTAACCAGGGGATGCT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUASP YFP Rab19 DN was a gift from Matthew Scott (Addgene plasmid # 37721 ; http://n2t.net/addgene:37721 ; RRID:Addgene_37721)

  • For your References section:

    Thirty-one flavors of Drosophila rab proteins. Zhang J, Schulze KL, Hiesinger PR, Suyama K, Wang S, Fish M, Acar M, Hoskins RA, Bellen HJ, Scott MP. Genetics. 2007 Jun;176(2):1307-22. Epub 2007 Apr 3. 10.1534/genetics.106.066761 PubMed 17409086
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