pUASP YFP Rab5 DN
(Plasmid
#37694)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 37694 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUASP
- Backbone size w/o insert (bp) 9939
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Vector typeInsect Expression ; Drosophila
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRab5
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SpeciesD. melanogaster (fly)
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MutationS43N (dominant negative)
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Entrez GeneRab5 (a.k.a. Dmel_CG3664, AAF51265, BAA88244, CG3664, DRab5, Dm Rab5, DmRab5, Dmel\CG3664, Drab5, RAB5, RAB5a, Rab, Rab 5, Rab-5, dRab5, drab5, l(2)k08232, rab5)
- Promoter GAL4
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Tag
/ Fusion Protein
- YFP (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer pUASp-3 5'-AGGTTTAACCAGGGGATGCT (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUASP YFP Rab5 DN was a gift from Matthew Scott (Addgene plasmid # 37694 ; http://n2t.net/addgene:37694 ; RRID:Addgene_37694) -
For your References section:
Thirty-one flavors of Drosophila rab proteins. Zhang J, Schulze KL, Hiesinger PR, Suyama K, Wang S, Fish M, Acar M, Hoskins RA, Bellen HJ, Scott MP. Genetics. 2007 Jun;176(2):1307-22. Epub 2007 Apr 3. 10.1534/genetics.106.066761 PubMed 17409086