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Addgene

pGL2-ptc-lu
(Plasmid #37678)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37678 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL2-Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5597
  • Vector type
    Insect Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ptc promoter (−758 to +130)
  • Species
    D. melanogaster (fly)
  • Promoter ptc
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer LucNrev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL2-ptc-lu was a gift from Philip Beachy (Addgene plasmid # 37678 ; http://n2t.net/addgene:37678 ; RRID:Addgene_37678)

  • For your References section:

    Nuclear trafficking of Cubitus interruptus in the transcriptional regulation of Hedgehog target gene expression. Chen CH, von Kessler DP, Park W, Wang B, Ma Y, Beachy PA. Cell. 1999 Aug 6;98(3):305-16. 10.1016/S0092-8674(00)81960-1 PubMed 10458606
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