pcDNA3-ShhN-Ren
(Plasmid #37677)

• Depositing Lab: Philip Beachy
• Publication: Ma et al Cell. 2002 Oct 4;111(1):63-75.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5446
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: shh
• Species: M. musculus (mouse)
• Mutation: soluble form (see comments)
• Entrez Gene: Shh (a.k.a. 9530036O11Rik, Dsh, HHG-1, Hhg1, Hx, Hxl3, M100081, ShhNC)
• Promoter: CMV
• Tag / Fusion Protein:
  • renilla luciferase (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: CMV-Fwd
• 3′ sequencing primer: BGH-Rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The ShhN construct contains a stop codon following the site of internal cleavage and therefore produces a protein (ShhN) containing the same amino acid residues as the processed protein but lacking cholesterol modification.

Renilla luciferase was inserted in-frame into the amino-terminal signaling domain located in a peripheral loop within the structure of ShhN.