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MXW-PGK-c-let-7-LP4A-IRES-EGFP
(Plasmid #37646)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37646 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MXW-PGK-IRES-EGFP
  • Backbone manufacturer
    Chang-Zheng Chen Lab
  • Backbone size w/o insert (bp) 7066
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    EGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    cel-let-7
  • Alt name
    loop mutant 4A
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    582
  • Mutation
    LP4 - AATATTACCA=>AATAAAACCA

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MXW-PGK-c-let-7-LP4A-IRES-EGFP was a gift from Chang-Zheng Chen (Addgene plasmid # 37646 ; http://n2t.net/addgene:37646 ; RRID:Addgene_37646)

  • For your References section:

    Loop nucleotides control primary and mature miRNA function in target recognition and repression. Yue SB, Trujillo RD, Tang Y, O'Gorman WE, Chen CZ. RNA Biol. 2011 Nov-Dec;8(6):1115-23. 10.4161/rna.8.6.17626 PubMed 22142974
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