FUGW-H1-GFP-neomycin
(Plasmid #37632)

• Purpose: (Empty Backbone)
• Depositing Lab: Valeri Vasioukhin
• Publication: Lien et al J Cell Biol. 2008 Dec 15. 183(6):989-97.

Backbone

• Vector backbone: FUGW
• Backbone manufacturer: David Baltimore (Addgene Plasmid #14883)
• Modifications to backbone: FUGW was modified by placing internal ribosome entry site (IRES) and neomycin sequences downstream from EGFP and cloning of human H1 promoter followed by unique BamHI–EcoRI sites upstream from the ubiquitin promoter
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: G418

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: H1
• 3′ sequencing primer: EGFP-N

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Subclone shRNA oligos into the unique BamHI-EcoRI sites downstream of the H1 promoter