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Addgene

FUGW-H1-GFP-neomycin
(Plasmid #37632)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37632 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FUGW
  • Backbone manufacturer
    David Baltimore (Addgene Plasmid #14883)
  • Modifications to backbone
    FUGW was modified by placing internal ribosome entry site (IRES) and neomycin sequences downstream from EGFP and cloning of human H1 promoter followed by unique BamHI–EcoRI sites upstream from the ubiquitin promoter
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    G418

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Subclone shRNA oligos into the unique BamHI-EcoRI sites downstream of the H1 promoter

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FUGW-H1-GFP-neomycin was a gift from Valeri Vasioukhin (Addgene plasmid # 37632 ; http://n2t.net/addgene:37632 ; RRID:Addgene_37632)

  • For your References section:

    Alpha-E-catenin binds to dynamitin and regulates dynactin-mediated intracellular traffic. Lien WH, Gelfand VI, Vasioukhin V. J Cell Biol. 2008 Dec 15. 183(6):989-97. 10.1083/jcb.200805041 PubMed 19075109
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