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Purpose(Empty Backbone)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 37631 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepmaxGFP
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Backbone manufacturerAmaxa
- Backbone size (bp) 2800
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Modifications to backboneGFP in pMAX-GFP vector was replaced with Gateway casette from pLenti6 V5 DEST (Invitrogen)
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Vector typeMammalian Expression
- Promoter CMV
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Tag
/ Fusion Protein
- V5 (C terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Kanamycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberUnknown
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMAX-DEST was a gift from Valeri Vasioukhin (Addgene plasmid # 37631 ; http://n2t.net/addgene:37631 ; RRID:Addgene_37631) -
For your References section:
A causal role for ERG in neoplastic transformation of prostate epithelium. Klezovitch O, Risk M, Coleman I, Lucas JM, Null M, True LD, Nelson PS, Vasioukhin V. Proc Natl Acad Sci U S A. 2008 Feb 12. 105(6):2105-10. 10.1073/pnas.0711711105 PubMed 18245377
Map uploaded by the depositor.
