pCXWB-EBNA1
(Plasmid #37624)

• Purpose: Non-replicating episomal expression of EBNA1, which enhances transfection efficiency and expression from episomal plasmids
• Depositing Lab: Shinya Yamanaka
• Publication: Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293.

Backbone

• Vector backbone: pCXWB
• Backbone size w/o insert (bp): 5078
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EBNA-1
• Alt name: HHV4-EBNA-1.1
• Species: human herpesvirus 4
• Insert Size (bp): 1926
• GenBank ID: 3783709
• Entrez Gene: EBNA-1 (a.k.a. HHV4_EBNA-1.1)
• Promoter: CAG

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pCAG-F
• 3′ sequencing primer: WPRE-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: EBNA-1 is from Invitrogen. CAG Promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J.
• Articles Citing this Plasmid:
  • 51 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry