p6900 PHAGE-P CMVT N-HA-hE6AP III C840A
(Plasmid
#37606)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 37606 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePHAGE-P-CMVt N-HA GAW
- Backbone size w/o insert (bp) 7800
- Total vector size (bp) 10400
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameE6AP III C840A
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SpeciesH. sapiens (human)
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Insert Size (bp)2616
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MutationC840A
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Entrez GeneUBE3A (a.k.a. ANCR, AS, E6-AP, EPVE6AP, HPVE6A, PIX1)
- Promoter CMV tet regulatable
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Tag
/ Fusion Protein
- HA (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p6900 PHAGE-P CMVT N-HA-hE6AP III C840A was a gift from Peter Howley (Addgene plasmid # 37606 ; http://n2t.net/addgene:37606 ; RRID:Addgene_37606) -
For your References section:
Identification and proteomic analysis of distinct UBE3A/E6AP protein complexes. Martinez-Noel G, Galligan JT, Sowa ME, Arndt V, Overton TM, Harper JW, Howley PM. Mol Cell Biol. 2012 May 29. 10.1128/MCB.00201-12 PubMed 22645313