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Addgene

pLenti-tkd2MetGFP
(Plasmid #37562)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37562 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLentiCMV GFP Puro
  • Backbone manufacturer
    Eric Campeau Addgene Plasmid 17448
  • Backbone size w/o insert (bp) 8608
  • Total vector size (bp) 9324
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MET
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    704
  • Mutation
    only contains cytoplasmic domain amino acids L1157-S1390
  • Entrez Gene
    MET (a.k.a. AUTS9, DA11, DFNB97, HGFR, RCCP2, c-Met)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer gacgccatccacgctgttttgacc
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-tkd2MetGFP was a gift from David Rimm (Addgene plasmid # 37562 ; http://n2t.net/addgene:37562 ; RRID:Addgene_37562)
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