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Addgene

pBSG01
(Plasmid #37558)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37558 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSY07
  • Backbone size w/o insert (bp) 6515
  • Total vector size (bp) 6822
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PreScission protease cleavage site-S tag-TEV site-Z
  • Species
    N/A
  • Insert Size (bp)
    332

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBSG01 was a gift from Wei-Lih Lee (Addgene plasmid # 37558 ; http://n2t.net/addgene:37558 ; RRID:Addgene_37558)

  • For your References section:

    A novel patch assembly domain in Num1 mediates dynein anchoring at the cortex during spindle positioning. Tang X, Germain BS, Lee WL. J Cell Biol. 2012 Mar 19;196(6):743-56. 10.1083/jcb.201112017 PubMed 22431751
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