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Addgene

pRS306:Met3p-mCherry-Tub1
(Plasmid #37554)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37554 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS306
  • Backbone size w/o insert (bp) 4381
  • Total vector size (bp) 7114
  • Vector type
    Bacterial Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Met3p-mCherry-Tub1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2700

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (destroyed during cloning)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS306:Met3p-mCherry-Tub1 was a gift from Wei-Lih Lee (Addgene plasmid # 37554 ; http://n2t.net/addgene:37554 ; RRID:Addgene_37554)

  • For your References section:

    Quantitative analysis of Pac1/LIS1-mediated dynein targeting: Implications for regulation of dynein activity in budding yeast. Markus SM, Plevock KM, St Germain BJ, Punch JJ, Meaden CW, Lee WL. Cytoskeleton (Hoboken). 2011 Mar;68(3):157-74. doi: 10.1002/cm.20502. Epub 2011 Feb 3. 10.1002/cm.20502 PubMed 21294277
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