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Addgene

pRS305:Met3p-mCherry-Tub1
(Plasmid #37553)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37553 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS305
  • Backbone size w/o insert (bp) 5504
  • Total vector size (bp) 8237
  • Vector type
    Bacterial Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Met3p-mCherry-Tub1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2700

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (destroyed during cloning)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS305:Met3p-mCherry-Tub1 was a gift from Wei-Lih Lee (Addgene plasmid # 37553 ; http://n2t.net/addgene:37553 ; RRID:Addgene_37553)

  • For your References section:

    Motor- and tail-dependent targeting of dynein to microtubule plus ends and the cell cortex. Markus SM, Punch JJ, Lee WL. Curr Biol. 2009 Feb 10;19(3):196-205. Epub 2009 Jan 29. 10.1016/j.cub.2008.12.047 PubMed 19185494
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