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Addgene

pCDNA3 IRF9-STAT2C
(Plasmid #37544)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37544 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IRF-9 STAT2 C
  • Species
    H. sapiens (human)
  • GenBank ID
  • Entrez Gene
    IRF9 (a.k.a. IRF-9, ISGF3, ISGF3G, p48)
  • Entrez Gene
    STAT2 (a.k.a. IMD44, ISGF-3, P113, PTORCH3, STAT113)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

C8Y point mutation in IRF9 should not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3 IRF9-STAT2C was a gift from Curt Horvath (Addgene plasmid # 37544 ; http://n2t.net/addgene:37544 ; RRID:Addgene_37544)

  • For your References section:

    A hybrid IRF9-STAT2 protein recapitulates interferon-stimulated gene expression and antiviral response. Kraus TA, Lau JF, Parisien JP, Horvath CM. J Biol Chem. 2003 Apr 11;278(15):13033-8. Epub 2003 Feb 5. 10.1074/jbc.M212972200 PubMed 12574168
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