pcDNA3.1 AK3 Hyg
(Plasmid
#37539)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 37539 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1(+) hygro
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5597
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAK3
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Alt nameadenylate kinase-3
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SpeciesH. sapiens (human)
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Entrez GeneAK3 (a.k.a. AK3L1, AK6, AKL3L, AKL3L1, FIX)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1 AK3 Hyg was a gift from David Sidransky (Addgene plasmid # 37539 ; http://n2t.net/addgene:37539 ; RRID:Addgene_37539) -
For your References section:
Adenylate kinase 3 sensitizes cells to cigarette smoke condensate vapor induced cisplatin resistance. Chang X, Ravi R, Pham V, Bedi A, Chatterjee A, Sidransky D. PLoS One. 2011;6(6):e20806. Epub 2011 Jun 15. 10.1371/journal.pone.0020806 PubMed 21698293