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Addgene

TagRFP-T-Rabenosyn-5
(Plasmid #37537)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37537 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    GFP-Rabenosyn5
  • Backbone manufacturer
    Corvera Lab
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 7044
  • Modifications to backbone
    TagRFP-T from Roger Tsien was used to replace the GFP in the Corvera Lab GFP-Rabenosyn5.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rabenosyn5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2354
  • GenBank ID
    40034008
  • Entrez Gene
    RBSN (a.k.a. Rabenosyn-5, ZFYVE20)
  • Promoter CMV
  • Tag / Fusion Protein
    • TagRFP-T (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Designed and prepared by Deborah Leonard, Deanna Navaroli and Anil Chawla (Corvera Lab)

There are a few sequence discrepancies between the full sequence and the Addgene QC sequence--these do not affect the function of the plasmid, but the XhoI site located at the 3' end of the TagRFP-T on the depositor's map is NOT present in the vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TagRFP-T-Rabenosyn-5 was a gift from Silvia Corvera (Addgene plasmid # 37537 ; http://n2t.net/addgene:37537 ; RRID:Addgene_37537)
  • For your References section:

    Rabenosyn-5 defines the fate of the transferrin receptor following clathrin-mediated endocytosis. Navaroli DM, Bellve KD, Standley C, Lifshitz LM, Cardia J, Lambright D, Leonard D, Fogarty KE, Corvera S. Proc Natl Acad Sci U S A. 2012 Feb 21;109(8):E471-80. Epub 2012 Jan 30. 10.1073/pnas.1115495109 PubMed 22308388