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Purposetemplate for PCR amplification of the monomer used for assembly of the DNA-binding tandem repeat region of the TALE.
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 37527 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePJ201
- Backbone size w/o insert (bp) 2644
- Total vector size (bp) 2746
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMonomer NH
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Alt nameRVD NH
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Alt namepNH_v2
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SpeciesSynthetic
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Insert Size (bp)102
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer No
- 3′ sequencing primer M13F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Monomer NH_V2 was a gift from Feng Zhang (Addgene plasmid # 37527 ; http://n2t.net/addgene:37527 ; RRID:Addgene_37527) -
For your References section:
Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Cong L, Zhou R, Kuo YC, Cunniff M, Zhang F. Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. 10.1038/ncomms1962 PubMed 22828628