Skip to main content
Addgene

FLAG-NLRC5
(Plasmid #37521)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37521 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCMV-Tag 2b
  • Backbone size w/o insert (bp) 4300
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NLRC5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5600
  • Entrez Gene
    NLRC5 (a.k.a. CLR16.1, NOD27, NOD4)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-NLRC5 was a gift from Thomas Kufer (Addgene plasmid # 37521 ; http://n2t.net/addgene:37521 ; RRID:Addgene_37521)

  • For your References section:

    A role for the human nucleotide-binding domain, leucine-rich repeat-containing family member NLRC5 in antiviral responses. Neerincx A, Lautz K, Menning M, Kremmer E, Zigrino P, Hosel M, Buning H, Schwarzenbacher R, Kufer TA. J Biol Chem. 2010 Aug 20;285(34):26223-32. Epub 2010 Jun 10. 10.1074/jbc.M110.109736 PubMed 20538593
Related items:
Commonly requested with: