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myc-NLRC5 iso3
(Plasmid #37510)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37510 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NLRC5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5600
  • Mutation
    isoform 3
  • Entrez Gene
    NLRC5 (a.k.a. CLR16.1, NOD27, NOD4)
  • Promoter CMV
  • Tag / Fusion Protein
    • myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The iso3 plasmid actually encodes the full cDNA (not only the ORF).
In comparison to the FL NLRC5, iso3 contains an insert that leads to a stop codon according to IPI00647281.1 .

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    myc-NLRC5 iso3 was a gift from Thomas Kufer (Addgene plasmid # 37510 ; http://n2t.net/addgene:37510 ; RRID:Addgene_37510)

  • For your References section:

    NLRC5 Controls Basal MHC Class I Gene Expression in an MHC Enhanceosome-Dependent Manner. Neerincx A, Rodriguez GM, Steimle V, Kufer TA. J Immunol. 2012 May 15;188(10):4940-50. Epub 2012 Apr 6. 10.4049/jimmunol.1103136 PubMed 22490867
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