pEV1.05
(Plasmid
#37498)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 37498 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEV1
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Backbone manufacturercustom
- Backbone size w/o insert (bp) 6700
- Total vector size (bp) 15961
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFBF-2::fbf-2 3’UTR
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SpeciesC. elegans (nematode)
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Insert Size (bp)9300
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Tag
/ Fusion Protein
- GFP-Tev(LAP) (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13_for20, GFP-F
- 3′ sequencing primer GFP-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEV1.05 was a gift from Geraldine Seydoux (Addgene plasmid # 37498 ; http://n2t.net/addgene:37498 ; RRID:Addgene_37498) -
For your References section:
The C. elegans homolog of nucleoporin Nup98 is required for the integrity and function of germline P granules. Voronina E, Seydoux G. Development. 2010 May;137(9):1441-50. Epub 2010 Mar 24. 10.1242/dev.047654 PubMed 20335358