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Addgene

pEV9.03
(Plasmid #37495)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37495 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pL4440gw
  • Backbone manufacturer
    Caldwell(?)
  • Backbone size w/o insert (bp) 2790
  • Modifications to backbone
    Exchanged ccdB gene in original backbone for insert
  • Vector type
    Worm Expression, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    npp-16
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    511
  • Mutation
    nts 162-673 of cds
  • Entrez Gene
    npp-16 (a.k.a. Y56A3A.17)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEV9.03 was a gift from Geraldine Seydoux (Addgene plasmid # 37495 ; http://n2t.net/addgene:37495 ; RRID:Addgene_37495)
  • For your References section:

    The C. elegans homolog of nucleoporin Nup98 is required for the integrity and function of germline P granules. Voronina E, Seydoux G. Development. 2010 May;137(9):1441-50. Epub 2010 Mar 24. 10.1242/dev.047654 PubMed 20335358