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Addgene

pEV1.02
(Plasmid #37487)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37487 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEV1
  • Backbone manufacturer
    custom
  • Backbone size w/o insert (bp) 6700
  • Total vector size (bp) 16371
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GLH-1::nos-2 3’UTR
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    9600
  • Tag / Fusion Protein
    • GFP-Tev(LAP) (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEV1.02 was a gift from Geraldine Seydoux (Addgene plasmid # 37487 ; http://n2t.net/addgene:37487 ; RRID:Addgene_37487)
  • For your References section:

    The C. elegans homolog of nucleoporin Nup98 is required for the integrity and function of germline P granules. Voronina E, Seydoux G. Development. 2010 May;137(9):1441-50. Epub 2010 Mar 24. 10.1242/dev.047654 PubMed 20335358