pRK5 Flag Grb10 wt long
(Plasmid #37481)

• Depositing Lab: David Sabatini
• Publication: Hsu et al Science. 2011 Jun 10;332(6035):1317-22.

Backbone

• Vector backbone: pRK5-FLAG
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Grb10
• Species: H. sapiens (human)
• Insert Size (bp): 1785
• Mutation: isoform a, 594aa
• GenBank ID: NM_005311.4
• Entrez Gene: GRB10 (a.k.a. GRB-IR, Grb-10, IRBP, MEG1, RSS)
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: SV40pA-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry