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Addgene

pBAD-D4
(Plasmid #37473)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37473 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 6000
  • Modifications to backbone
    Before BamHI site in pBAD there is the His tag of pRSETB
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    D4 second generation cameleon (calcium sensor)
  • Alt name
    D family cameleon
  • Species
    Synthetic
  • Insert Size (bp)
    1950
  • GenBank ID
  • Promoter Ara

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBAD Forward
  • 3′ sequencing primer pBAD Reverse
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Constructed by Amy Palmer in the lab of Roger Tsien
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Genetically encoded, ratiometric, calcium biosensor that contains enhanced CFP and citrine. D4 does not bind wild-type calmodulin. It has a Kd' of 195 microM.

See also Palmer & Tsien (2006) Nature Protocols.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD-D4 was a gift from Amy Palmer & Roger Tsien (Addgene plasmid # 37473 ; http://n2t.net/addgene:37473 ; RRID:Addgene_37473)

  • For your References section:

    Ca2+ indicators based on computationally redesigned calmodulin-peptide pairs. Palmer AE, Giacomello M, Kortemme T, Hires SA, Lev-Ram V, Baker D, Tsien RY. Chem Biol. 2006 May;13(5):521-30. 10.1016/j.chembiol.2006.03.007 PubMed 16720273
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