pBAD-D2
(Plasmid #37470)

• Depositing Labs: Amy Palmer, Roger Tsien
• Publication: Palmer et al Chem Biol. 2006 May;13(5):521-30.

Backbone

• Vector backbone: pBAD
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 6000
• Modifications to backbone: Before BamHI site in pBAD there is the His tag of pRSETB
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: D2 second generation cameleon (calcium sensor)
• Alt name: D family cameleon
• Species: Synthetic
• Insert Size (bp): 1950
• GenBank ID:
• Promoter: Ara

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pBAD Forward
• 3′ sequencing primer: pBAD Reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Constructed in the lab of Roger Tsien by Amy Palmer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Genetically encoded, ratiometric, calcium biosensor that contains enhanced CFP and citrine. D2 does not bind wild-type calmodulin. It has a Kd1' and Kd2' of 0.8 microM and 28 microM.

See also Palmer & Tsien (2006) Nature Protocols.