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Purpose(Empty Backbone)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 37464 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepPD95.75
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Backbone manufacturerAndrew Fire (Addgene plasmid # 1494)
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Modifications to backboneThe GFP gene of pPD95.75 was replaced by the Venus gene optimized for C. elegans codon usage.
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Vector typeWorm Expression
- Promoter none
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13-Rev (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe backbone plasmid (pPD95.75) was a gift from Andrew Fire.
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPD95.75-wVenus was a gift from Hitoshi Sawa (Addgene plasmid # 37464 ; http://n2t.net/addgene:37464 ; RRID:Addgene_37464) -
For your References section:
Multiple Wnts redundantly control polarity orientation in Caenorhabditis elegans epithelial stem cells. Yamamoto Y, Takeshita H, Sawa H. PLoS Genet. 2011 Oct;7(10):e1002308. Epub 2011 Oct 13. 10.1371/journal.pgen.1002308 PubMed 22022276
