pAAV-EF1a-DIO-HB
(Plasmid
#37452)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 37452 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backboneAAV
- Total vector size (bp) 5300
-
Vector typeAdeno-associated vector
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)Stbl3
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameHistone, 2A element, rabies B19 glycoprotein
-
Alt namehistone
-
Alt name2A element
-
Alt namerabies B19 glycoprotein
-
Insert Size (bp)2700
- Promoter EF1a
-
Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsrG1/EcoRV (destroyed during cloning)
- 3′ cloning site NheI/XbaI (destroyed during cloning)
- 5′ sequencing primer EF1a_fwd (Common Sequencing Primers)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that there are 2 mutations in Histone, D26G and V119I. These mutations should not affect plasmid function.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAAV-EF1a-DIO-HB was a gift from Edward Callaway (Addgene plasmid # 37452 ; http://n2t.net/addgene:37452 ; RRID:Addgene_37452)