pLentiN 16E6no*
(Plasmid #37445)

• Purpose: Lentiviral expression of HPV 16E6no* in mammalian cells
• Depositing Lab: Karl Munger
• Publication: Spangle et al J Virol. 2012 May 2.

Backbone

• Vector backbone: pLenti 6.3/V5 DEST
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 9387
• Total vector size (bp): 8623
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HPV 16E6no*
• Alt name: HPV 16E6
• Alt name: HpV16gp1
• Alt name: E6
• Species: HPV
• Insert Size (bp): 542
• Mutation: V42L
• GenBank ID: NC_001526.2 NP_041325.1
• Entrez Gene: E6 (a.k.a. HpV16gp1)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Flag (N terminal on insert)
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: V5 reverse

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry