pB-actin 18E6
(Plasmid
#37434)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 37434 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepB-actin
- Backbone size w/o insert (bp) 7500
- Total vector size (bp) 7977
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameHPV 18E6
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Alt name18E6
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Alt nameE6
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SpeciesHPV
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Insert Size (bp)477
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GenBank IDNC_001357.1 NP_040310.1
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Entrez GeneE6 (a.k.a. HpV18gp1)
- Promoter Human B-actin
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer 18E6 fwd
- 3′ sequencing primer Bactin 3' MCS seq110rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pB-actin 18E6 was a gift from Karl Munger (Addgene plasmid # 37434 ; http://n2t.net/addgene:37434 ; RRID:Addgene_37434) -
For your References section:
Activation of cap dependent translation by mucosal Human Papillomavirus E6 proteins is dependent on the integrity of the LXXLL binding motif. Spangle J, Ghosh-Choudhury N, Munger K. J Virol. 2012 May 2. 10.1128/JVI.00487-12 PubMed 22553330