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Addgene

pB-actin 16E6no*
(Plasmid #37433)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37433 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pB-actin
  • Backbone size w/o insert (bp) 7500
  • Total vector size (bp) 8230
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    HPV 16E6no*
  • Alt name
    HPV 16E6
  • Alt name
    HpV16gp1
  • Alt name
    E6
  • Species
    HPV
  • Insert Size (bp)
    456
  • Mutation
    V42L
  • GenBank ID
    NC_001526.2 NP_041325.1
  • Entrez Gene
    E6 (a.k.a. HpV16gp1)
  • Promoter Human B-actin

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer B-actin 16E6
  • 3′ sequencing primer Bactin 3' MCS seq110rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pB-actin 16E6no* was a gift from Karl Munger (Addgene plasmid # 37433 ; http://n2t.net/addgene:37433 ; RRID:Addgene_37433)

  • For your References section:

    Activation of cap dependent translation by mucosal Human Papillomavirus E6 proteins is dependent on the integrity of the LXXLL binding motif. Spangle J, Ghosh-Choudhury N, Munger K. J Virol. 2012 May 2. 10.1128/JVI.00487-12 PubMed 22553330
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