Cre Stoplight 2.4
(Plasmid #37402)

• Depositing Lab: Thomas Hughes
• Publication: Yang et al Biotechniques. 2001 Nov;31(5):1036, 1038, 1040-1.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4273
• Total vector size (bp): 5241
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Cre Stoplight 2.4
• Alt name: Stoplight
• Insert Size (bp): 2023
• Promoter: CMV
• Tags / Fusion Proteins:
  • ZsGreen1 (N terminal on insert)
  • mCherry (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1, EcoRV etc. (unknown if destroyed)
• 3′ cloning site: loxp (unknown if destroyed)
• 5′ sequencing primer: CAG enhancer
• 3′ sequencing primer: Amp

Resource Information

• Supplemental Documents:
  • Cre Stoplight 2.4
  • Annotated GenBank file
• Addgene Notes:
  • Addgene Diagnostic Digest
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Cre Stoplight 2.4 differs from the original Cre Stoplight described in the associated publication (Biotechniques. 2001 Nov;31(5):1036, 1038, 1040-1.). See the supplemental document Cre Stoplight 2.4 above for more information.

Please note that Addgene digest and NGS results suggest that the plasmid contains two mCherry ORFs in tandem. This is not expected to affect plasmid function as the second mCherry should not be expressed from the plasmid.