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Addgene

pCaShs-egghead (zw4)
(Plasmid #37400)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37400 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCaSpeR-hs
  • Backbone size w/o insert (bp) 8780
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    egghead
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    egh (a.k.a. Dmel_CG9659, CG9659, Dmel\CG9659, EG:BACR25B3.8, ZW-4, l(1)3Ae, l(1)zw4, zw-4, zw4)
  • Promoter Hsp70

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer pCasper-hs (5'-GCAACTACTGAAATCTGCCAAG-3')
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCaShs-egghead (zw4) was a gift from Norbert Perrimon (Addgene plasmid # 37400 ; http://n2t.net/addgene:37400 ; RRID:Addgene_37400)

  • For your References section:

    The neurogenic genes egghead and brainiac define a novel signaling pathway essential for epithelial morphogenesis during Drosophila oogenesis. Goode S, Melnick M, Chou TB, Perrimon N. Development. 1996 Dec;122(12):3863-79. PubMed 9012507
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