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pCaSpeR-4 porcupine (genomic DNA)
(Plasmid #37376)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37376 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCaSpeR-4
  • Backbone size w/o insert (bp) 7855
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    porcupine
  • Alt name
    porc
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    5500
  • Entrez Gene
    por (a.k.a. Dmel_CG6205, 15175, CG6205, Dmel\CG6205, Porc, dmPorc, l(1)17Ac, poc, porc)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Perrimon Lab plasmid #123

The EcoRI-XbaI genomic DNA covering the porc locus was cloned in pCaspeR4 to generate a genomic porc rescue construct.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCaSpeR-4 porcupine (genomic DNA) was a gift from Norbert Perrimon (Addgene plasmid # 37376 ; http://n2t.net/addgene:37376 ; RRID:Addgene_37376)

  • For your References section:

    The segment polarity gene porcupine encodes a putative multitransmembrane protein involved in Wingless processing. Kadowaki T, Wilder E, Klingensmith J, Zachary K, Perrimon N. Genes Dev. 1996 Dec 15;10(24):3116-28. 10.1101/gad.10.24.3116 PubMed 8985181
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