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Addgene

pEGFP-N1 gp78fl
(Plasmid #37375)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37375 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gp78
  • Alt name
    AMFR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1931
  • Mutation
    P179L and F262L
  • GenBank ID
    11724934
  • Entrez Gene
    AMFR (a.k.a. GP78, RNF45, SPG89)
  • Tag / Fusion Protein
    • EYFP (C terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-N1 gp78fl was a gift from Allan Weissman (Addgene plasmid # 37375 ; http://n2t.net/addgene:37375 ; RRID:Addgene_37375)

  • For your References section:

    The tumor autocrine motility factor receptor, gp78, is a ubiquitin protein ligase implicated in degradation from the endoplasmic reticulum. Fang S, Ferrone M, Yang C, Jensen JP, Tiwari S, Weissman AM. Proc Natl Acad Sci U S A. 2001 Dec 4. 98(25):14422-7. 10.1073/pnas.251401598 PubMed 11724934
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