pT7-EGFP-C1-HsPAN3
(Plasmid
#37374)
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 37374 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepEGFP-C1
-
Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4759
- Total vector size (bp) 6823
-
Modifications to backboneT7-promoter upstream of the EGFP-gene.
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameHsPAN3
-
Alt namePAN3
-
SpeciesH. sapiens (human)
-
Insert Size (bp)2064
-
Entrez GenePAN3 (a.k.a. RP11-179F17.3)
- Promoter CMV
-
Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pT7-EGFP-C1-HsPAN3 was a gift from Elisa Izaurralde (Addgene plasmid # 37374 ; http://n2t.net/addgene:37374 ; RRID:Addgene_37374) -
For your References section:
GW182 proteins directly recruit cytoplasmic deadenylase complexes to miRNA targets. Braun JE, Huntzinger E, Fauser M, Izaurralde E. Mol Cell. 2011 Oct 7;44(1):120-33. 10.1016/j.molcel.2011.09.007 PubMed 21981923