pT7-EGFP-C1-HsNot3
(Plasmid #37372)

• Depositing Lab: Elisa Izaurralde
• Publication: Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4780
• Total vector size (bp): 7039
• Modifications to backbone: T7-promoter upstream of the EGFP-gene
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HsNot3
• Alt name: CNOT3
• Species: H. sapiens (human)
• Insert Size (bp): 2259
• GenBank ID: NM_014516
• Entrez Gene: CNOT3 (a.k.a. LENG2, NOT3, NOT3H)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry