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pFLAG-EEF1D (pcDNA 3.1+) LG3-1
(Plasmid #37364)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37364 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1+
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 6300
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EEF1D
  • Alt name
    elongation factor 1-delta
  • Alt name
    EEF1D transcript variant 2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    880
  • GenBank ID
    gi|25453472
  • Entrez Gene
    EEF1D (a.k.a. EF-1D, EF1D, FP1047)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFLAG-EEF1D (pcDNA 3.1+) LG3-1 was a gift from David Litchfield (Addgene plasmid # 37364 ; http://n2t.net/addgene:37364 ; RRID:Addgene_37364)

  • For your References section:

    Unbiased functional proteomics strategy for protein kinase inhibitor validation and identification of bona fide protein kinase substrates: application to identification of EEF1D as a substrate for CK2. Gyenis L, Duncan JS, Turowec JP, Bretner M, Litchfield DW. J Proteome Res. 2011 Nov 4;10(11):4887-901. Epub 2011 Oct 13. 10.1021/pr2008994 PubMed 21936567
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