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Myc-CK2beta 6KR (pAB1016)
(Plasmid #37361)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37361 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRC/CMV
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5542
  • Total vector size (bp) 6315
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CK2beta 6KR
  • Alt name
    CK2β
  • Alt name
    Casein Kinase II beta
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    773
  • Mutation
    K33R, K139R, K177R, K191R, K208R, K212R
  • Entrez Gene
    CSNK2B (a.k.a. CK2B, CK2N, CSK2B, Ckb1, Ckb2, G5A, POBINDS)
  • Promoter CMV-F
  • Tag / Fusion Protein
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Myc-CK2beta 6KR (pAB1016) was a gift from David Litchfield (Addgene plasmid # 37361 ; http://n2t.net/addgene:37361 ; RRID:Addgene_37361)

  • For your References section:

    Development of a stabilized form of the regulatory CK2beta subunit that inhibits cell proliferation. French AC, Luscher B, Litchfield DW. J Biol Chem. 2007 Oct 5;282(40):29667-77. Epub 2007 Aug 6. 10.1074/jbc.M706457200 PubMed 17681943
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