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Addgene

NLS-GAI(1-92)
(Plasmid #37317)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37317 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pECFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GAI
  • Species
    A. thaliana (mustard weed)
  • Mutation
    contains aa 1-92
  • Entrez Gene
    GAI (a.k.a. AT1G14920, F10B6.34, F10B6_34, GAI PROTEIN, GIBBERELLIC ACID INSENSITIVE, RESTORATION ON GROWTH ON AMMONIA 2, RGA2)
  • Promoter CMV
  • Tag / Fusion Protein
    • NLS (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsrGI (unknown if destroyed)
  • 3′ cloning site SacI (unknown if destroyed)
  • 5′ sequencing primer CMV-F, EGFP-C
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The nuclear localization signal was inserted into the CFP-GAI(1-92) plasmid using the NheI/AgeI restriction sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NLS-GAI(1-92) was a gift from Takanari Inoue (Addgene plasmid # 37317 ; http://n2t.net/addgene:37317 ; RRID:Addgene_37317)

  • For your References section:

    Rapid and orthogonal logic gating with a gibberellin-induced dimerization system. Miyamoto T, Derose R, Suarez A, Ueno T, Chen M, Sun TP, Wolfgang MJ, Mukherjee C, Meyers DJ, Inoue T. Nat Chem Biol. 2012 Mar 25;8(5):465-70. doi: 10.1038/nchembio.922. 10.1038/nchembio.922 PubMed 22446836
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