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Addgene

pCaShs-Tum
(Plasmid #37303)

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Item Catalog # Description Quantity Price (USD)
Plasmid 37303 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCaShs-hopscotch
  • Backbone manufacturer
    Addgene plasmid# 37299
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hopTum-1
  • Alt name
    Tumorous-lethal
  • Alt name
    Tum-1
  • Species
    D. melanogaster (fly)
  • Mutation
    Tul-l mutation is a G to A transversion at nucleotide 1641 resulting in the substitution of a Glu for a Gly at amino acid 341
  • GenBank ID
    AAA62441.1
  • Entrez Gene
    hop (a.k.a. Dmel_CG1594, 4, CG1594, Dm JAK, DmHD-160, Dmel\CG1594, HD-160, HOP, Hop, Hop1, Hop2, Hpo, JAK, Jak, L4, Tum, Tum-1, d-jak, hop/JAK, jak, l(1)10Be, l(1)G18, l(1)L4, l(1)hop, msvl)
  • Promoter Hsp70

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer pCasper-hs (5'-GCAACTACTGAAATCTGCCAAG-3')
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Perrimon Lab plasmid #101

The pCaShs-Tum construct was generated by the replacement of a 220 bp SacII-BstEII fragment from pCaShs-hop (Addgene plasmid# 37299) with the same fragment of PCR-generated DNA from hopTum-1 hemizygous flies.

This mutation is a G to A transversion at nucleotide 1641 resulting in the substitution of a Glu for a Gly at amino acid 341.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCaShs-Tum was a gift from Norbert Perrimon (Addgene plasmid # 37303 ; http://n2t.net/addgene:37303 ; RRID:Addgene_37303)

  • For your References section:

    Activation of a Drosophila Janus kinase (JAK) causes hematopoietic neoplasia and developmental defects. Harrison DA, Binari R, Nahreini TS, Gilman M, Perrimon N. EMBO J. 1995 Jun 15;14(12):2857-65. PubMed 7796812
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